Food allergy and anaphylaxis – 2046. Cloning and characterization of the gene for acidic thaumatin-like protein, an important allergen from sapodilla plum (manilkara zapota)
نویسندگان
چکیده
Methods A case of oral allergy syndrome to sapodilla and custard apple was investigated following approval by Institutional Ethics Committee. Sapodilla allergy was confirmed by diagnostic tests (SPT and allergen-specific IgE). Sapodilla proteins were separated on SP-Sepharose by adsorption at pH 4 followed by step elution at pH 5 (SP1), and with increasing NaCl – 0.1 M (SP2) and 0.2 M (SP3). Forward primers and nested reverse primers specific to the SP1 component were designed based on its N-terminal sequence and conserved regions of homologous plant TLPs. PCR was performed using sapodilla (cv. cricket ball) leaf genomic DNA as template.
منابع مشابه
Basic and clinical immunology – 3033: Structural analyses and allergencity predicition of sapodilla (Manikara zapota) acidic thaumatin-like protein by bioinformatics
Background Oral allergy syndrome to sapodilla plum is caused by a 21 kD allergen existing as two isoforms: an acidic thaumatin-like protein (TLP-1) and a basic thaumatin-like protein (TLP-2). The composite sequence of TLP-1 comprising the N-terminal sequence 1-7 (A-T-F-D-V/I-V-N-) and the deduced sequence 8-207 (GenBank JN624813.1) was used for this analysis. The major objective of this study w...
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